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By William K. Wang

ISBN-10: 0824702484

ISBN-13: 9780824702489

Refines and optimizes key positive aspects of the 1st edition-with new illustrative case experiences. Examines benefits and drawbacks of utilizing typical and new membrane applied sciences analyzes a variety of functions, together with the main up to date telephone tradition harvesting and product strategy validation thoughts and extra.

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About an order of magnitude greater amounts of BSA were found to adsorb on polysulfone and polyamide membranes, compared with less hydrophobic CA membranes [108], while another study reported no detectable adsorption of BSA and transferrin on hydrophilic regenerated-cellulose membranes [26]. , in surface roughness), even if they are made of the same polymer [108,109]. Adsorption of proteins usually leads to a reduction of the membrane hydraulic and diffusive permeabilities, and hence to a decline in the magnitude of transmembrane fluxes and ECS Starling flow.

26] were more than an order of magnitude lower than the BSA concentrations, as is typical for these two proteins in most protein-containing media for cell culture [38,76]. The steady-state distributions of transferrin for different amounts of BSA in the ECS (Figure 11) were found to bear a close resemblance to the distributions of BSA itself (see also Figure 10; note that different reactor geometries were used in [25] and [26]). This can be easily explained by the facts that osmotic pressure had the same effect on the distribution of either protein in the ECS; and that transferrin, because of its relatively low concentration, contributed only negligibly to the osmotic effects.

The presence of the osmotic pressure derivative term required that the above equation be solved numerically. The obtained distribution of uL was used to evaluate the local ECS velocities, uS and vS, which were then inserted into eq. (17), and the latter was also solved numerically. Because of the coupling between the HFBR hydrodynamics and ECS protein distribution, the solutions of eqs. (17) and (18) were iterated at each new time level until convergence was attained. A subsequent study by Patkar et al.

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Membrane Separations in Biotechnology by William K. Wang


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