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Download e-book for iPad: Drag Racing Picture Book by Tim Mitchelle

By Tim Mitchelle

Are you or your kids enthusiasts of racing, race automobiles, autos or drag racing. ;This e-book is stuffed with dozens of images of drag races and drag race vehicles. turn via this publication to discover amazing photographs of cool race automobiles on each one web page. this can be the precise e-book for somebody who loves vans, machines, race autos, and firetrucks.

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Extra info for Drag Racing Picture Book

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This is determined by assaying zero-dose tubes (Table II) with serial dilutions of rabbit anti-AFP serum in the presence of working concentration of tracer. The proper antibody dilution is at mid-point on the resulting tracer bind- 24 ONCOFETAL PROTEINS [2] ing curve. Rabbits almost invariably produce high titer high-affinity antiAFP sera, typically used in the assay at a dilution of 1 : 50,000 or less. AFP Standards. Preparations of pure AFP are quantitated by the Lowry method against bovine serum albumin (Sigma, Fraction V).

Working Tracer. 6 A~00unit in zerodose (maximum binding) tubes]. The proper amount of tracer is determined by assaying zero-dose tubes (Table II) with serial dilutions of stock tracer in the presence of a large excess of first antibody. 44oo unit after 15 min). In the present system this amounts to about 10 ng of tracer AFP per assay tube, assuming that the stock tracer contains all of the 500/zg of AFP used for conjugation (considering the sensitivity of the assay, this relatively high concentration of tracer points to a substantial loss of AFP antigenic sites occurring during conjugation).

For instance, absorbance readings and calculation of results can be reduced to minutes with the use of an automated spectrophotometer and a computer program correlating AFP concentrations and absorbance values. 4 A fast centrifugal enzyme analyzer can monitor kinetics of substrate reaction and integrate absorbance readings, calculation of results and data output. 5 The pellet washing step remains a limitation. Measuring free instead o f bound tracer clearly would simplify the assay, improve its precision, and increase its potential for complete automation.

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Drag Racing Picture Book by Tim Mitchelle


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